人类腺病毒 呼肠孤病毒和单纯疱疹病毒在Lα细胞上的致病变效应

The Cytopathogenic Effect of Human Adenovirus, Reovirus and Herpes Simplex Virus on Lα-cell

转脊髓灰质炎（脊灰）病毒受体基因的鼠肺Ｌα细胞，只能增殖脊灰病毒，不能增殖其它肠道病毒。它的应用对脊灰病毒的分离不失为一便捷、省时、经济和可靠的方法。但是在应用过程中也发现，在急性弛缓性麻痹（ＡＦＰ）病例粪便或直肠拭子标本中，也可能存在人类腺病毒、呼肠孤病毒和单纯疱疹病毒等。这些病毒在Ｌα细胞上可产生病变，这种情况偶有发生，且病变形态具有一定的特征，但不影响Ｌα细胞在分离脊灰病毒中的应用。因此，我们建议如分离物在Ｌα上产生病变，也应对其进行认真定型后才能作出是否是脊灰病毒的结论。

On Lα-cell, the poliovirus can replicate but the non-polio enteroviruses (NPEV) can not. The Lα-cell is the transgenic mouse lung cells established by the Japanese scientist S. Koike, on the membrane of these cells the poliovirus receptors are bound. This is a reliable, rapid and convenient way for poliovirus isolation and, besides, unexpensive. But in the course of laboratory practicing, we found that human adenovirus, reovirus and herppes simplex virus which probably may exist in fecal samples or rectal swabs from AFP cases can also induce cytopathogenic effect(CPE)on Lα-cell, though it occurred only occassionally. The way to distinguish them from poliovirus is by their characteristic CPE and their low replicating titers in Lα-cell. But, anyhow, we suggest that the CPE inducing isolates in Lα-cell had better be typed so as to confirm whether the isolate is poliovirus or not.