儿童再生障碍性贫血病毒病因学的初步研究

Prellmlllary study on the viral role In the pathogenesls of childhood aplastic anemia.

用PCR和ELISA技术对38例AA患儿的外周血进行了B 19、EBV、CMV及HBV系列检测。结果:(1)病例组B 19-DNA阳性9例(23.7％),与正常对照比较差异有非常显著性意义(P=0.00871),EBV-DNA阳性1例,HBsAg和HBeAg同时阳性者2例,上述病毒的总阳性率为31.6％(12/38例)。(2)将B 19阳性9例按AAA和CAA进一步比较,两者无显著性差异(P=0.673)。提示:(1)近1/3AA病例的发生可能与上述病毒感染有关。而B 19较其他病毒与AA的关系更为密切。(2)B 19感染与AAA和CAA的发生均有关。(3)HBV仍为我国HAAA的重要病因,EBV可能为儿童获得性AA的病毒病因之一。

Using PCR and ELISA skills, B 19-DNA, EBV-DNA, CMV-DNA and five serum markers of HBV were detected in 38 children with aplastic anemia (AA). The results showed that positive B 19-DNA was observed on 9 cases (23. 7%)in the AA group and none in the control group (P=0. 0087). Positive EBV-DNA and HBV-antigens were detected on 1 case and 2 cases, respectively. The total positive rate of these three viruses was 31- 6% (12/38). No significant statistically difference between acute AA and chronic AA was observed on all 9 AA cases with positive B 19-DNA (P = 0. 673). It is suggested that! a) The pathegenesis of 1/3 AA cases were probably correlated to viruses infection, specially B 19 infection, b) The pathegenesis of acute AA and Chronic AA were all correlated to B 19. c) HBV remains the important reason responsible for hepatitis-associated AA in China. It is possible that EBV may be one of causes in childhood acquired AA.