环境砷中毒人群皮肤损害与DNA氧化损伤的关联性

Association between skin lesions and 8-OHdG among a chronic arsenic exposure population

选择内蒙古自治区某县长期暴露于高砷饮用水的人群中有皮肤损害者199人(病例组)和无皮肤损害者91人(对照组)作为研究对象,检测饮用水砷、尿砷和DNA氧化损伤的主要标志物8-羟基脱氧鸟苷(8-OHdG)水平,以分析慢性砷中毒人群DNA氧化损伤与皮肤损害的相关性.结果表明,砷性皮肤损害组和无皮肤损害组人群尿中8-OHdG水平的中位数(四分位间距)分别为4.9(3.5～6.3)μg/g和5.3(4.1～7.2)μg/g,2组对象尿中8-OHdG水平间差异无统计学意义(P=0.074);多元Logistic回归分析结果显示,在校正了年龄、体质指数(BMI)、吸烟、饮酒和饮用高砷水时间等可能的混杂因素后,皮肤损害组人群尿中8-OHdG水平升高的比值比(OR)为1.1(95%CI:0.6,1.9),提示在砷暴露人群中,有无皮肤损害与8-OHdG水平的差异无统计学意义(P=0.863).慢性砷中毒人群皮肤损害与体内的DNA氧化损伤之间无显著的关联性,无皮肤损害人群同样有DNA氧化损伤的风险.

The association between skin lesions and DNA oxidative damage among a chronic arsenic exposure population was studied by investigating 199 cases with skin lesions and 91 without skin lesions（control group）.Concentrations of inorganic arsenic（iAs） in urinary,monomethylarsonic acid（MMA）,dimethylarsinic acid（DMA）,and 8-hydroxydeoxyguanine（8-OHdG） were measured.Multiple logistic regression was performed to discover the association between skin lesions and DNA oxidative damage.Among participants with skin lesions and without skin lesions,median（interquartile range） of 8-OHdG concentrations in urine were 4.9μg/g creatinine（3.5～6.3） and 5.3（4.1～7.2）μg/g creatinine,respectively.There was no significant difference in 8-OHdG concentrations between case and control group（P=0.074）.After adjusting for potential confounding factors,such as age,body mass index（BMI）,cigarette smoking,alcohol consumption,and the number of years to high arsenic contaminated water,the results of multiple logistic regression showed that the odds ratio（95% confidence interval,95% CI） of 8-OHdG in cases with skin lesions was 1.1（0.6,1.9） compared with participants without skin lesions.Skin lesions did not cause significantly increase of 8-OHdG concentrations（P=0.863）.There was no significant association between skin lesions and DNA oxidative damage in an arsenic exposure population in Inner Mongolia.