摘要
采用经口摄食染毒,探讨全氟辛烷磺酸(PFOS)对大鼠血清与脑组织中PFOS浓度及海马细胞内钙离子浓度([Ca2+]i)的影响,观察大鼠大脑皮质、海马和小脑组织病理学变化.结果表明,染毒组大鼠血清与脑组织中PFOS浓度、海马细胞内[Ca2+]i均显著高于对照组,且随着染毒剂量的升高,海马细胞内[Ca2+]i升高.大鼠血清与脑组织中PFOS浓度、脑组织中PFOS浓度与海马细胞[Ca2+]i均呈现显著的正相关关系.染毒组大鼠大脑皮质、海马和小脑组织中未见尼氏体染色变浅等病理性改变.
Effect of subchronic perfluorooctane sulfonate（PFOS） exposure on free calcium level（[Ca^2＋]i） in hippocampus in rats and the mechanism of the neurotoxicity induced by PFOS was studied.The adult male Wistar rats were treated with oral PFOS exposure at dosage of 2,8,32,128mg/kg,respectively.After 60 days＇ exposure,the rats were sacrificed,and then single-cell suspension of hippocampus was prepared.[Ca^2＋]i was determined with Fura-2/AM fluorescence probe.The brain and serum were concentrated by liquid–phase extraction and then the concentration of PFOS was determined using HPLC/MS.The cortex,hippocampus and cerebellum were collected for evaluation of histopathology change.PFOS concentration in serum and brain in PFOS treated groups was significantly higher than that in the control group（P〈0.01）.There was a significant positive correlation between [Ca^2＋]i and PFOS concentration in serum and brain.No obvious change was observed in the morphology of the brain in rats exposed to PFOS.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2010年第1期110-115,共6页
China Environmental Science
基金
国家自然科学基金资助项目(30771772)
