摘要
本文介绍以 GA_4-BSA 结合物诱导制得兔抗 GA_4-Me 抗体的赤霉素放射免疫测定法及其药盒的特征和应用。该抗体与 GA_1,GA_3,GA_4和 GA_7有较高的亲和反应,而与 GA_5、GA_7与 GA_(20)的结合能力甚小。本法检定 GA_8范围在25～800pg/50μl 之间。对吸胀大麦种子的 GA_8测定结果表明,α-淀粉酶活性的诱导与种子内源 GA_1,GA_3无关,但伴随着 GA_(4+7)含量的升高而增大。
A radioimmunoassay kit specific for the detection of gibberellin A_1, A_3, A_4andA_7 as low as 25 pg in partially purified plant extracts with the antiserum againstgibbcrellin A_4-BSA conjugats elicited in rabbits was developed.The serum showed high-er specificity with gibberellin A_1,A_3,A_4 and A_7,while much lower with A_5,A_7 and A_(20) respectively.~3H-GA_4 methyl ester as radioactive antigen and GA_4 methylester as the standard (measuring range 25～800 pg) were used in this assay.The inductionof α-amylase activity during imbibition of barley seeds had nothing to do withendogenous GA_1,GA_3 but increased with the increase of GA_(4+7) contents inside thesecds.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1990年第2期24-27,共4页
Journal of Nanjing Agricultural University
关键词
赤霉素
放射免疫测定
药盒
Gibberellins
radioimmunoassay
barley
α-amylase
