中国水牛小型肉孢子虫的研究

STUDIES ON THE SMALL SARCOCYST-FORMING SPECIES IN CHINESE BUFFALOS

以自然感染微型包囊的肉孢子虫的水牛食道6务,切碎,混匀,分别感染仔猫5只,幼犬6只。结果所有犬均获得感染,排出卵囊或孢子囊。潜隐期为9～17天。所有猫在试验期内没有排出卵囊或孢子囊。以试验犬获得的孢子囊2.8×10~5,6.2×10~4及1.5×10~5个分别感染水牛犊2头及乳牛犊1头。2头水牛犊感染后均表现严重生长不良,1头于第60天扑杀,淋巴结触片发现裂殖体样虫体。另一头于102天扑杀,食道肌肉内有微型包囊。但1头乳牛犊和对照牛(1头水牛犊,1头乳牛犊)生长良好,组织学检查未发现虫体。作者根据其为微型包囊,感染犬获得成功、回归黄牛没有成功,初步认为是李氏肉孢子虫 Sarcocystis levinei。

Six dogs and five cats were fed microcysts of Sarcocystis from naturally infectedChinese buffaloes oesophageal muscles.All experimental dogs started shedding sporulat-cd oocyst or sporocystis in the feces.The prepatent period were 9～17 days.All experi-mental cats did not shed oocyst or sporocystis during the experimental period.The Chinese buffalo calves and a bull calt fed 2.8×10~5,6.2×10~4and 1.5×10~5 sporocysts respectively from experimental infected dogs.Two bufalo calves werestunted in growth.A bufflo calf had schizont-like in the renal lymphaden 60 days afterexperimental infection,the other buffalo calf had microcyst in oesophageal muscles after102 days,but a bull calt,a control buffalo and a control calt had no schizont-like ormicrocyst.These findings suggest that the microcyst belongs to Sarcocyst levinei.