不同转移潜能的人肿瘤细胞系金属蛋白酶活性分析

Zymographic analysis of MMP s in human carcinoma cell lines with different metastasis potential

目的探讨不同转移潜能的人类肿瘤细胞的金属蛋白酶（ＭＭＰｓ）活性与其侵袭转移潜能的相关性。方法分别选取具有不同转移潜能的人类肺癌细胞系（ＰＧ、ＰＡａ、ＢＥ１、ＣＬ３、ＬＨ７）、黑色素瘤细胞系（ＷＭ３５、ＷＭ１３４１ｂ、ＷＭ９８３ａ、ＷＭ４５１）及前列腺癌细胞系（ＰＣ，ＰＣ３Ｍ），经细胞培养，条件培养基的收集与浓缩，利用明胶Ｚｙｍｏｇｒａｐｈｙ法检测以上各组细胞ＭＭＰ２和ＭＭＰ９的产生及活性差异，并将这种差异与各自的转移潜能联系起来。结果转移能力相对较高的细胞系产生ＭＭＰｓ的能力相应强于转移能力相对较低者：ＰＧ高于ＰＡａ，ＢＥＩ高于ＣＬ３和ＬＨ７。在进展期黑色素瘤株ＷＭ９８３ａ和转移瘤株ＷＭ４５１出现了ＭＭＰ９的表达，早期瘤株则无。前列腺瘤细胞ＰＣ３的转移性克隆ＰＣ３Ｍ的条件培养基中有较高的ＭＭＰ９活性。结论肿瘤细胞的侵袭转移潜能与其产生ＭＭＰｓ的能力密切相关。

Objective To investigate the correlation between matrix metalloproteinases (MMPs) activities and metastatic potential of several groups of human carcinoma cells with different metastatic potential.Methods Several groups of human carcinoma cell lines (human lung carcinoma、human prostatic carcinoma and melanoma) with different metastatic potential were selected. By using cell culture、collection and concentration of conditioned media and zymographic analysis method the difference of MMPs production and activity among those cell lines were detected. Results The MMPs production capabilities of carcinoma cells rose following the increase of their invasive and metastatic potentials: that of PG is much higher than PAa′s, and BE1 is higher also than CL3 and LH7. Advanced stage melanoma cell WM983a and WM451 product MMP 9, but primary stage cells don′t. There are MMP 9 in the conditioned medium of prostatic carcinoma cell PC 3M, the metastatic clone of PC 3 which don′t express MMP 9. Conclusion The expression of MMPs especially MMP 9 of carcinoma cells is closely correlated to the metastatic and invasive potential.