胶体金免疫层析技术快速定量检测相思子毒素

Rapid and quantitative detection of abrin using colloid gold immunochromatographic assay

目的:建立胶体金免疫层析技术快速定量检测相思子毒素。方法:利用胶体金标记和双抗体夹心免疫层析技术,建立相思子毒素的快速检测方法,评价其特异性和敏感性,并拟合检测曲线进行定量检测。在面粉、饼干、奶粉、苹果汁、牛奶、果冻等食品样品中添加相思子毒素模拟污染样品,评价该方法对固体、半固体、液体等食品样品的检测能力。结果与结论:该法可在15min内完成定性和半定量检测,灵敏度为30ng/ml,线性范围30～600ng/ml、回收率80%～110%,变异系数小于15%。所建立的检测相思子毒素的胶体金免疫层析方法,能快速、灵敏、特异、准确地检测样品中的相思子毒素,并可实现定量,适用于现场快速检测。

Objective：To develop a rapid and quantitative detection method for abrin using colloid gold immunochromatographic assay.Methods：The rapid detection method was established with double-antibody sandwich assay.Quantification was realized by constructing a standard curve.The specificity and sensitivity of the method were tested,and its feasibility was evaluated by various abrin-added food samples.Results and Conclusion：This established method could accomplish qualitative and semi-quantitative detection in 15 minutes ; the sensitivity was up to 30 ng/ml with a linear range from 30 ng/ml to 600 ng/ml. The recovery rate was 80% - 110%, and the variation coefficient was less than 15%. The colloidal gold immunochromatographic assay is rapid, specific, sensitive,accurate and suitable for field detection.