BALB/c和昆明小鼠Aβ侧脑室注射建立阿尔茨海默病小鼠模型比较

Comparison of Alzheimer's disease animal model in BALB/c and Kunming mice by intracerebroventricular injection of β-amyloid

目的:选择合适品系的小鼠通过侧脑室注射β-淀粉样蛋白片段25～35(Aβ25～35)建立阿尔茨海默病(Alzheimer’s disease,AD)小鼠模型。方法:采用跳台、Morris水迷宫实验对比了BALB/c小鼠、昆明(KM)小鼠侧脑室注射Aβ25～35后学习记忆能力的变化,筛选出用于适合制备AD动物模型的小鼠品系。结果:正常KM小鼠与BALB/c小鼠比较,被动回避反应能力无明显差异,而KM小鼠的空间学习记忆能力明显强于BALB/c小鼠。侧脑室注射Aβ25～35后,雌性和雄性BALB/c小鼠和雌性KM小鼠学习记忆能力与各自正常对照组相比无明显变化;雄性KM小鼠与其正常对照组相比,在Morris水迷宫实验中逃避潜伏期明显延长、第一次穿环时间增加而穿环次数明显减少。结论:侧脑室注射Aβ25～35后,雄性KM小鼠Morris水迷宫实验的学习记忆能力降低明显优于雌性KM小鼠及雌性、雄性BALB/c小鼠,是制备AD小鼠模型比较理想的动物。

Objective：To compare the Alzheimer s disease model in two species of mice by intracerebroventricular injection of β-amyloid peptide 25-35（Aβ25-35）.Method：The step down test and Morris water-maze were used to investigate the influence of the mice s learning and memorizing ability after intracerebroventricular injection of β-amyloid.Results：Normal Kunming mice and BALB/c mice had no significant difference in step down test,but the space cognitive ability of Kunming mice was better than that of BALB/c mice.The learning response in step-down test and Morris water-maze is no influence in male, female BALB/c mice and female Kunming mice. Marked differences were observed in male Kunming mice in acquisition, performance and reversal of a place learning response in a Morris water-maze. Conclusion：The male Kunming mice is much better than female Kunming mice and male, female BALB/c mice in preparing Alzheimer＇ s disease animal model by intracerebroventricular injection of Aβ25-35 , and is the most suitable animal in this experiment.