运动干预对胰岛素抵抗大鼠骨骼肌IL-1β表达的影响

Effect of Exercise on the Interleukin-1 beta(IL-1β) Expression in Muscle Tissue of Insulin-Resistant Rats

目的观察运动干预对高脂饲料诱导胰岛素抵抗(IR)大鼠白细胞介素1β(IL-1β)表达的影响,探讨运动减轻IR的可能机制。方法健康Wistar雄性大鼠分为基础饲料喂养组(normal chow group,NC),高脂膳食喂养组(high-fat diet group,HF)。高脂膳食喂养Wistar雄性大鼠10周,构建IR动物模型。10周后,HF组再随机分为高脂喂养运动组和非运动组,游泳运动干预4周。游泳运动干预前后以正常血糖-高血浆胰岛素钳夹实验技术[hyperinsulinemic-euglycemic clamp(HEC)technique]评估IR大鼠胰岛素敏感性,ELISA法测定大鼠血清IL-1β水平,RT-PCR法测定大鼠骨骼肌IL-1βmRNA表达。结果HF组大鼠葡萄糖输注率(glucose infusion rate,GIR)显著低于NC组(P<0.05),HF组血清IL-1β水平及骨骼肌组织IL-1βmRNA表达明显高于NC组(P<0.05,P<0.01);运动组大鼠血清IL-1β水平及骨骼肌组织IL-1βmRNA表达明显低于非运动组(P<0.05),与NC组差异无显著性(P>0.05)。结论运动改善IR大鼠胰岛素敏感性,可能与降低IR大鼠IL-1β的表达有关。

Objective To investigate the effect of swimming exercise on insulin sensitivity and interleukin-1 beta（IL-1β）mRNA expression in muscle tissue of insulin-resistant（IR） rats induced by high-fat diet.Methods The IR rat model was induced by high-fat diet feeding for 10 weeks and assessed by hyperinsulinemic-euglycemic clamp technique（HEC）. The rats were divided into the fllowing three groups： normal group without exercise,IR groups with and without swimming exercise.After 4 weeks swimming excersie,insulin sensitivity and serum IL-1β levels were measured.Results Glucose infusion rate（GIR） of the IR rats was significantly elevated than that of non-exercise IR rats（P〈0.01）.Moreover,serum IL-1β levels were reduced markedly in IR rats（P〈0.01）.Conclusion High-fat diet feeding can partly reverse IR of rats to normal value.Swimming exercise improves insulin sensitivity and reduces the levels of IL-1β in IR rats induced by high-fat diet.