鼻腔、鼻窦鳞状细胞癌中p53、p21蛋白表达及HPV16/18感染的意义

Significances of p53,p21 expression and human papilloma virus 16/18 infection in sinonasal squamous cell carcinomas

目的探讨p53和p21的表达及HPV感染在鼻腔、鼻窦鳞状细胞癌(简称鼻鳞癌)中的意义。方法用EnVision免疫组化二步法检测28例鼻鳞癌和24例鼻息肉中p53和p21的表达情况,并用原位杂交方法检测其HPV16/18的感染情况。结果鼻鳞癌组中p53的阳性表达率为64.29%,鼻息肉组中p53未见表达,两组比较差异极显著(P<0.01);而p21的阳性表达率在鼻鳞癌组和鼻息肉组间差异不显著;鼻鳞癌组HPV16/18检出率为78.57%,鼻息肉组HPV16/18检出率为0,两组比较差异极显著(P<0.01)。结论p53基因突变以及HPV感染与鼻鳞癌的发生、发展可能有一定的相关性。

Objective To investigate the significance of the expression of p53 and p21 and HPV infection in sinonasal squamous cell carcinoma （referred to as nasal SCC）. Methods 28 cases of nasal SCC and 24 cases of nasal polyps were stained for p53 and p21 by immunohistochemical EnVision method, and detection of HPV 16/18 gene by in situ hybridization, respectively. Results The expression rate of p53 was 64.29% in nasal SCC group, and was negative in nasal polyp group, with significantly different （P 〈 0.01）. The positive expression rate of p21 in the two groups had no significant difference （P = 0.407）. The positive rate of HPV 16/18 in nasal SCC group was 78.57%, but was negative in nasal polyps, with significantly different（ P〈0.01）. Conclusion p53 gene mutation and HPV infection might have some relevance with the occurrence and development of nasal SCC.