先天性尿道下裂阴茎皮肤细胞雌雄激素受体表达及胞核内的聚集

Androgen and Estrogen Receptors in Prepuce of Children with Hypospadias

目的：探索先天性尿道下裂与阴茎皮肤细胞雄激素受体（ＡＲ）、雌激素受体（ＥＲ）的表达及细胞核内的聚集的关系。方法：通过受体的放射配基结合分析，对２８例不同程度的先天性尿道下裂患儿外生殖器皮肤细胞内的ＡＲ、ＥＲ的表达及细胞内的分布进行了测定。结果：实验显示尿道下裂患儿外生殖器皮肤细胞内及胞核内ＡＲ量较正常对照减少（Ｐ＜０．０１），尿道下裂的严重程度与细胞内及细胞核内的ＡＲ量无关（Ｐ＞０．０５）。细胞内总ＥＲ与正常无显著性差异（Ｐ＞０．０５），胞核内ＥＲ量较正常对照减少（Ｐ＜０．０５）。结论：ＡＲ异常是先天性尿道下裂的原因之一，先天性尿道下裂也属于雄激素不敏感综合征的范畴。

Objective: To study the relationship between androgen receptors(AR) and estrogen receptors(ER) in cells and nuclei of prepuce in patients with hypospadias and the severity of hypospadias. Methods: AR and ER expression and its distribution in the preputial cells of 28 cases with different types of hypospadias were examined by radioligand binding assay(RBA). Results: The total cellular and nuclear AR concentrations in prepuce of hypospadias patients were lower than those of control group (P<0.01). There was no correlation between the severity of hypospadias and the cellular and nuclear levels of AR (P>0.05). There was no statistically significant difference in cellular ER between the hypospadias patients and the control group (P>0.05). The nuclear ER in hypospadias patients was lower than that of control (P<0.05). Conclusions: Abnormality of AR is a contributing factor in the pathogenesis of hypospadias. Hypospadias may be a manifestation of androgen insensibility syndrome. Reduced level of nuclear ER may be a secondary change.