艾滋病患者T淋巴细胞表面归巢分子的表达在抗病毒治疗后升高

Significant increasing of homing molecules expression on T lymphocytes in AIDS patients after treatment with HAART

目的探讨艾滋病（AIDS）患者高效抗反转录病毒治疗（HAART）前后T淋巴细胞表面归巢分子CD49d、CCR9、CD62I．表达的变化情况。方法采用流式细胞术检测42例艾滋病患者和18例HIV阴性健康对照的外周血T淋巴细胞表面CD49d、CCR9和CD62L表达，用BDFACSDiva软件分析计算各组细胞表达的百分率。结果治疗后组外周血的平均CD4^＋T淋巴细胞明显高于治疗前组（P〈0．01）；治疗前组CD3^＋CD49d^＋、CD3^＋CCR9^＋、CD3^＋CD62L^＋、CD3^＋CD4^＋、CD4^＋CD49d^＋、CD4^＋CCR9^＋、CD4^＋CD62L^＋、CD8^＋CD49d^＋、CD8^＋CD62L^＋T淋巴细胞的百分率显著低于治疗后组和阴性对照组（P〈0．05）；CD3^＋CD8^＋T淋巴细胞的百分率高于治疗后组（P〈0．05）。治疗后组CD3^＋CCR9^＋、CD8^＋CCR9^＋、CD8^＋CD62L^＋T淋巴细胞的百分率均低于阴性对照组（P均〈0．001）。结论AIDS患者外周血T淋巴细胞亚群不仅比例失调，而且其表面表达肠道归巢分子CD49d、CCR9，淋巴结归巢分子CD62L的数量发生异常改变。抗病毒治疗可以逆转以上部分免疫病理变化。建议肠道归巢分子CD49d、CCR9和淋巴结归巢分子CD62L可作为艾滋病疾病进展和评价机体HAART后免疫重建的指标。

Objective To evaluate the homing molecules expression of CD49d, CCR9, CD62L on T lymphoeytes in AIDS patients before and after treatment with HAART. Methods The study was performed in 42 cases of AIDS patients and 18 cases of healthy controls. The expression of CD49d, CCR9 and CD62L on T lymphocytes in AIDS patients and healthy controls were analyzed by flow cytometry. Software BD FACSDiva was used to calculate the percentage of expression. Results The number of peripheral CD4^＋ T lymphocytes in group on-HAART was significantly increased compared with group pre-HAART （P 〈 0.01） ; the frequency of CD3^＋ CD49d^＋ , CD3^＋ CCR9^＋ , CD3^＋ CD62L^＋ , CD3^＋ CD4^＋ , CD4^＋ CD49d^＋ , CD4^＋ CCR9^＋ , CD4^＋ CD62L^＋ , CD8^＋ CD49d ^＋ , CD8 ^＋ CD62L^＋ T lymphocyte in group pre-HAART were statistically decreased compared with group on-HAART and controls （ P 〈 0.05 ） ; The frequency of CD3^＋ CD8 ^＋ T lymphocytes was significantly increased compared with group on-HAART（ P 〈 0.05） ; the frequency of CD3 ^＋ CCR9 ^＋ , CD8 ^＋ CCR9 ^＋ , CD8 ^＋ CD62L^＋ T lymphocytes in group on-HAART were significantly decreased than controls （ P 〈 0. 001 ）. Conclusion Not only the number of T lymphocytes sub-group, but the expression rate of gut homing molecules CD49d and CCR9, lymph node homing molecule CD62L on T lymphocytes was changed in AIDS patients ： the lower expression frequency of gut homing molecules CD49d and CCR9, lymph node homing molecule CD62L. Anti-virus therapy could partially reverse the immunologic pathological phenomena. CD49d, CCR9 and CD62L may be suggested to indicate the progression of AIDS and immunologic reconstitution after HAART.