FHIT基因转染对胆管癌细胞增殖与侵袭的影响

FHIT gene transfection inhibits the proliferation and invasiveness of human cholangiocarcinoma QBC939 cells

目的:探讨FHIT基因对胆管癌细胞的增殖与侵袭的影响.方法:将含FHIT基因的重组真核表达质粒转染入胆管癌细胞株QBC939,采用MTT实验检测转染前后细胞增殖活性,Transwell小室侵袭实验检测肿瘤细胞侵袭力.结果:转染后QBC939细胞的MTT吸光度明显下降(P<0.05),并且转移至小室滤膜下的细胞数明显减少(48±7vs109±14,104±12,均P<0.01).结论:FHIT基因能抑制胆管癌细胞株QBC939的增殖并降低其侵袭力.

AIM： To investigate the impact of fragile histidine triad （FHIT） gene transfection on the proliferation and invasiveness of human cholangiocarcinoma QBC939 cells. METHODS： The recombinant FHIT eukaryotic expression plasmid was introduced into QBC939 cells by liposome-mediated transfection. Cell proliferation was tested by methyl thiazolyl tetrazolium （MTT） assay. Cell invasion was determined by Transwell chamber assay. RESULTS： MTT assay showed that FHIT gene transfection significantly decreased the proliferation of QBC939 cells （P 〈 0.05）. Transwell chamber assay showed that the number of transfected cells that passed the Transwell membrane was significantly less than those of blank control and normal control cells （48 ± 7 vs 109 ± 14 and 104 ± 12, respectively; both P 〈 0.01）. CONCLUSION： FHIT gene can inhibit the proliferation and invasiveness of human cholangiocarcinoma QBC939 cells.