全血RUNX3启动子甲基化对胃癌早期诊断的意义

Significance of RUNX3 promoter methylation in DNA prepared from whole blood for early diagnosis of gastric carcinoma

目的:探讨全血RUNX3启动子甲基化对胃癌早期诊断的意义.方法:采用甲基化特异性聚合酶链反应(MSP)法,检测80例胃癌患者的癌组织及其对应的癌旁组织及全血中RUNX3启动子甲基化情况.结果:胃癌组织标本中RUNX3基因启动子甲基化率为45%,癌旁组织中甲基化率为0%,全血中甲基化率为40%.胃癌组织、全血中RUNX3基因启动子甲基化,差异无统计学意义(P>0.05).全血标本中RUNX3启动子甲基化表达情况与分化程度、是否有淋巴结转移密切相关,与年龄、性别、肿瘤部位浸润深度无关.结论:全血RUNX3启动子区甲基化对胃癌早期诊断有重要指导意义,与胃癌的发生、发展密切相关.

AIM： To investigate the significance of human runt-related transcription factor 3 （RUNX3） promoter methylation in DNA prepared from whole blood for early diagnosis of gastric carcinoma. METHODS： Methylation-specific PCR （MSP） was used to detect the methylation status of RUNX3 promoter in DNA prepared from carcinoma tissue, adjacent tissue and whole blood samples taken from 80 gastric cancer patients. RESULTS： The detection rates of RUNX3 promoter methylation in DNA prepared from gastric carcinoma tissue, adjacent tissue and whole blood samples were 45%, 0% and 40%, respectively. No significant difference was noted in the detection rate of RUNX3 promoter methylation between gastric carcinoma tissue and whole blood samples （P 〉 0. 05）. The RUNX3 promoter methylation status in gastric carcinoma was correlated with tumor differentiation and lymph node metastasis, but not with age, gender and tumor site. CONCLUSION： RUNX3 promoter methylation status in DNA prepared from whole blood is closely associated with the oncogenesis and metastasis of gastric carcinoma and has important significance for early diagnosis of gastric carcinoma.