胸腺细胞对胸腺基质细胞生长及功能的调节

The regulatory effect of thymocytes on the growth and functions of thymic stromal cells

目的研究胸腺细胞对不同亚群胸腺基质细胞生长及功能的调节作用。方法胸腺基质细胞增殖以３Ｈ－ＴｄＲ掺入法测定，ＩＬ－７活性以促ＩＬ－７依赖株法检测，其ｍＲＮＡ表达以ＲＴ－ＰＣＲ法检测。结果胸腺细胞与ＭＴＳＣ４细胞分别以８０∶１，４０∶１或２０∶１的比例共育时，能明显抑制ＭＴＳＣ４细胞的增殖，而培养上清中ＩＬ－７活性无明显改变；当两种细胞的比例为１０∶１或５∶１时，则对ＭＴＳＣ４细胞的增殖无任何影响，而培养上清中的ＩＬ－７活性明显升高。ＲＴ－ＰＣＲ证实，与胸腺细胞共育的ＭＴＥＣ１和ＭＴＳＣ４细胞，ＩＬ－７ｍＲＮＡ水平明显提高。结论胸腺细胞能促进ＭＴＥＣ１和ＭＴＳＣ４细胞分泌ＩＬ－７。

Objective To investigate the regulatory effect of thymocytes on proliferation and function of different types of murine thymic stromal cells. Methods The proliferation of stromal cells was determined by 3 H TdR incorporation.IL 7 activity was assayed by the proliferation of IL 7 dependent cell line and IL 7 mRNA expression was analyzed by RT PCR. Results When thymocytes and MTSC4 were cocultured with cell ratio of 80∶1,40∶1 or 20∶1,the proliferation of MTSC4 were significantly inhibited,while the IL 7 activity in supernatants was significantly increased. RT PCR analysis showed that IL 7 mRNA expression in MTEC1 was significantly increased after cocultured with thymocytes. Conclusions Thymocytes could promote IL 7 production of MTEC1 and MTSC4, they had different regulatory effect on the proliferation and function of different types of stromal cells.