检测丙型肝炎患者NS5区抗体的临床意义探讨

Detection of antibody to HCV NS5 and its clinical significance

目的检测慢性丙型肝炎患者和１４例ＨＣＶ原发感染后ＮＳ５抗体长达一年半的动态变化，探讨ＮＳ５抗体的临床意义。方法应用重组ＮＳ５抗原，建立ＥＩＡ方法进行检测。结果慢性丙型肝炎患者抗－ＮＳ５抗体阳性率为６０．４８％，ＨＣＶ感染后一月抗－ＮＳ５抗体阳性率为１６．３５％，三月为７５％。结论抗－ＮＳ５抗体无早期诊断价值。抗－ＮＳ５抗体持续阳性者，血清ＡＬＴ多明显升高，抗－ＮＳ５抗体与肝脏疾病活动性相关。丙型肝炎患者中存在抗－Ｃ、抗－ＮＳ３、抗－ＮＳ４抗体阴性，而抗－ＮＳ５抗体单独阳性。

Objective To detect the antibodies to HCV NS5 as well as HCV C,NS3 and NS4 in 115 cases of patients with chronic hepatitis C and 115 serial blood samples of 14 cases of post transfusion hepatitis C. Methods The recombinant HCV NS5 antigen was used to establish EIA to capture the anti HCV NS5 antibody. Results We discovered that there were a few chronic hepatitis C patients (HCV RNA positive) and acute hepatitis C patients who were anti C, NS3 and NS4 Ab negative,while only anti NS5 Ab was positive.The dynamic changes of anti NS5 Ab were correlated with the serum ALT in most patients,indicating that anti NS5 Ab may reflect liver damage and disease activity to some extent. Conclusions The results show that detection of antibody to HCV NS5 has a unique value in HCV diagnosis.