摘要
以罗非鱼头为原料,初步探讨了硫酸软骨素的提取工艺,采用稀碱提取,酶解,三氯乙酸沉淀蛋白,乙醇沉淀的方法,考察了碱提取过程中碱浓度、料液比、温度、时间对提取率的影响,在单因素实验的基础上,利用正交实验进行了优化。得出了在碱浓度1%、料液比1∶5、提取温度50℃、提取时间7h,最大的得率为3.757%。
The articles introduced a method of preparation chondroitin sulfate form tilapia, skull the effects of alkali condition, ratio of solid to liquid, temperature and time on the extraction rate during the alkali extraction was studied by using alkali extraction, enzyme hydrolysis, the precipitation of proein by trichioroacetic acid and of chondroitin sulfate by ethanol, on the basis of single-factor tests these parameter of extraction process was optimized by orthogonal test. The results showed that the alkali concentration was 1%, the ratio of powdered shull of tilapia to alkali was 1：5, tempeture was 50 ℃ and the extraction of dilute alkali was 7 h, the extraction percent of chondroitin sulfate conld be improved up to 3.757%.
出处
《食品科技》
CAS
北大核心
2010年第1期235-238,共4页
Food Science and Technology
基金
国家科技支撑计划项目(2007BAD76B06)
关键词
罗非鱼
硫酸软骨素
碱提取
优化
Tilapia
Chondroitin sulfate
alkali extraction
optimization
