草鱼鱼鳔胶原性质的研究

Characterization of Collagens from Swim Bladders of Grass Carp

为了有效利用鱼类废弃物,从草鱼鳔中提取酸溶性胶原(ASC)和酶溶性胶原(PSC)并对其部分性质进行研究。ASC和PSC的提取得率以干基计分别为4.7%和11.1%;ASC和PSC的紫外光谱表明,最大吸收峰都接近于230nm;氨基酸组成分析表明,ASC和PSC中羟脯氨酸和羟赖氨酸的总含量分别为16.87%和17.55%;十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)图谱显示草鱼鳔胶原至少由两条不同的α链(α1和α2)组成,与I型胶原相似;ASC和PSC热变性温度(Td)分别为33.0、34.8℃,比猪皮胶原的热变性温度(37℃)低3～4℃。结果表明,草鱼鳔胶原有可能替代或作为补充陆生动物胶原广泛应用于工业领域。

In order to make more effective use of fish waste materials,acid-soluble and pepsin-soluble collagens（ASC and PSC） were extracted from swim bladders of grass carp and properties of ASC and PSC were investigated.The yields of ASC and PSC were 4.7% and 11.1%,respectively,on the basis of dry weight.UV spectra of ASC and PSC from grass carp exhibited the maximum absorption peak at 230 nm.Amino acid profiles of ASC and PSC revealed that contents of hydroxyproline and proline were 16.87% and 17.55%,respectively.SDS-PAGE patterns indicated that both ASC and PSC were composed of at least two different α-chains（α1 and α2）,which was similar to porcine type I collagen.The denaturation temperatures（Td） of ASC and PSC were 33.0 ℃ and 34.8 ℃,respectively,about 3-4 ℃ lower than that of porcine skin collagen（37 ℃）.These results suggested that collagens from swim bladders of grass carp had potential as an alternative or a supplementary source of collagen from land vertebrates being used in different fields for industrial purposes.