构建具有大麦脂转移蛋白1分泌能力的酿酒酵母

Construction of Saccharomyces cerevisiae Strain with Barley Lipid Transfer Protein 1 Secretory Capability

为了增强纯生啤酒的泡沫性能,从酿酒酵母表达质粒YEplac181出发,将大麦脂转移蛋白1(LTP1)成熟肽的编码序列置于酿酒酵母ADH1启动子(alcohol dehydrogenase promoter)和CYC1终止子(cytochrome C terminator)的调控下,构建大麦脂转移蛋白1的酿酒酵母表达质粒YEp181-KAMLC。通过酿酒酵母α-信息素信号肽的引导分泌,酿酒酵母表达的成熟大麦LTP1被分泌到发酵液中。对发酵液的检测表明,在发酵132h后LTP1的产量可达到29.45mg/L。

In order to enhance the foam stability of green draft beer,barley lipid transfer protein 1（LTP1） encoding DNA sequence from Hordeum vulgare was modified to construct yeast Saccharomyces cerevisiae expression plasmid YEp181-KAMLC of barley LPT1 using the plasmid YEplac181.The expression of barley LTP1 was regulated by alcohol dehydrogenase promoter（ADH1 promoter） and terminator cytochrome C terminator（CYC1） of Saccharomyces cerevisiae,and the matured form of barley LTP1 was secreted under the lead of α-mating factor 1 signal peptide.Fermentation for 132 h at 30 ℃ gave a yield of barley LTP1 as high as 29.45 mg/L.