补料分批发酵生产谷胱甘肽的研究

Fed-batch Fermentation of Glutathione

考察5L发酵罐中分批补加葡萄糖对发酵生产谷胱甘肽(GSH)的影响。采用20g/L初糖质量浓度,在发酵12h至27h每隔3h分别补加22、24、24、24、24g/L和22g/L葡萄糖,可以使酿酒酵母在发酵33h时GSH质量浓度达到72.49mg/L,细胞干质量浓度达到28.52g/L,分别为初糖20g/L分批培养方式的2.86倍和4.93倍。补料分批发酵可以明显促进酿酒酵母生长和提高GSH的合成。

In order to obtain high glutathione（GSH） yield and dry cell weight,fed-batch fermentation in a 5 L fermentor was adopted for glutathione production by a DL-ethionine,zinc chloride-resistant mutant of Saccharomyces cerevisiae.Strategy for fed-batch feeding of glucose at an initial concentration of 20 g/L was investigated.Results showed that the fed-batch feeding strategy of adding 22,24,24,24,24 g/L and 22 g/L glucose at intervals of 3 h from 12 to 27 h of fermentation resulted in 72.49 mg/L GSH concentration and 28.52 g/L dry cell weight,which was 2.86 times and 4.93 times as high as those obtained by single batch fermentation.This meant that fed-batch fermentation could remarkably promote strain growth and enhance GSH production.