环磷酰胺对蚕豆根尖细胞微核率的影响

Effect of Cyclophosphamide on Micronucleus Rate in Vicia faba Root Tips

研究用环磷酰胺作蚕豆根尖微核实验阳性对照的适宜浓度和结果判断指标。结果表明:用环磷酰胺作为蚕豆根尖微核实验的阳性对照,其浓度≤40.0mg/L时所致蚕豆根尖微核率具有较好的重现性和剂量-效应关系;微核指数在1.5以上可以认为实验样本具有致突变作用。0.8～10.0mg/L的环磷酰胺可作为蚕豆根尖微核实验的阳性对照,可用微核指数评价实验结果是否为阳性。该指标可以使蚕豆根尖微核实验的结果判定更准确、快速,具有重要的实际使用价值。

Objective：To study the optimal concentration of cyclophosphamide and evaluation standard of micronucleus tests in Vicia faba root tips.Methods：Micronucleus tests were conducted in Vicia faba root tips treated with different concentrations of cyclophosphamide.Results：Micronucleus rate in Vicia faba root tips exhibited a good reproducibility and dose-effect relationship at the concentration of cyclophosphamide below 40.0 mg/L.Mutagenesis of tested samples was observed when micronucleus index was over 1.5.Conclusion：Concentrations of cyclophosphamide between 0.8 mg/L and10.0 mg/L could be used as the positive control and micronucleus index could be used to assay mutagenicity due to its accuracy,rapidness and practical value.