天麻糖蛋白GGE2b的分离纯化及对急性血瘀大鼠血液流变的影响

Isolation,Purification and Hemorrheological Effect of Glycoprotein from Gastrodia elata (GGE2b) in Blood Stasis Rats

目的:从天麻中分离纯化得到糖蛋白组分GGE2b,研究其对急性血瘀大鼠血液流变学指标的影响。方法:采用离子交换和快速蛋白液相层析色谱(FPLC)技术分离天麻水溶性提取物,得到组分GGE2b。采用皮下注射盐酸肾上腺素结合冷刺激的方法制造急性血瘀大鼠模型。观察GGE2b各剂量组对急性血瘀大鼠全血黏度、血浆黏度、红细胞压积、红细胞聚集指数和红细胞变形指数的影响。结果:离子交换结合FPLC技术分离纯化得到天麻糖蛋白组分GGE2b。GGE2b60、120mg/kg能够显著降低急性血瘀模型大鼠高切、中切的全血黏度(P<0.05或0.01)和血浆黏度(P<0.01),降低红细胞聚集指数(P<0.05或0.01),增大红细胞变形指数(P<0.05)。结论:层析技术用于天麻糖复和物的分离纯化是可行的,且得到的糖蛋白组分GGE2b对急性血瘀大鼠异常的血液流变有良好的治疗效果。

Objective：To isolate and purify glycoprotein from Gastrodia elata（GGE2b） and investigate its hemorrheological effect in acute blood stasis rats.Methods：Soluble extracts of Gastrodia elata were purified by DEAE-Sepharose column chromatography and fast protein liquid chromatography（FPLC） to obtain GGE2b.Acute blood stasis rat model was developed by hypodermic adrenaline injection combined with cold-shock treatment.Totally 60 male rats were randomly divided into 6 groups of 10 rats each,namely saline group,acute blood stasis model group,PGE2b administration groups at doses of 30,60 and 120 mg/kg,and positive control group administered with Danshen injection.Effect of GGE2b on whole-blood viscosity,plasma viscosity,red blood cell（RBC） accumulation,RBC aggregation index and RBC deformability index in acute blood stasis rats was examined.Results：GGE2b was successfully purified from Gastrodia elata through combined technology of DEAE-Sepharose column chromatography and FPLC.Moreover,GGE2b administration at the doses of 60 mg/kg and 120 mg/kg in rats resulted in a dramatic decrease of plasma viscosity（P 0.05）,whole-blood viscosity（P 0.01） and RBC aggregation index（P 0.05）,and increase of RBC deformability index（P 0.05）.Conclusion：It is feasible to purify glycoproteins from Gastrodia elata using chromatography technology.GGE2b can result in a significant improvement of abnormal hemorrheology in rats with acute blood stasis.