再论MUG-Indole大肠杆菌检查法的研究

Rediscussion on MUG-Indole Method for Detection of E.coli in Pharmaceutical Products

首次应用４甲基伞形酮βＤ葡萄糖醛酸苷（４ＭｅｔｈｙｌｕｍｂｅｌｉｆｅｒｙｌβＤｇｌｕｃｕｒｏｎｉｄｅ，简称ＭＵＧ）检定药品中大肠杆菌获得成功，但有６％以上的大肠杆菌ＭＵＧ为阴性反应，故提出ＭＵＧ－Ｉｎｄｏｌｅ法，可将ＭＵＧ阳性、Ｉｎｄｏｌｅ阳性的大肠杆菌一次检出；对ＭＵＧ阴性、Ｉｎｄｏｌｅ阳性或ＭＵＧ阳性、Ｉｎｄｏｌｅ阴性的大肠杆菌辅以ＥＭＢ及ＩＭＶｉＣ试验，成功地解决了假阴性和假阳性的问题，提高了本法的准确性。

Methylumbellyferyl β D glucuronide(MUG)is firstly used to detect E.coli in pharmaceutical products.More than 6 % of the E.coli,however,do not be checked out with MUG.The MUG-Indole(M—I)assisted with IMViC test,a new method,could once check out the E.coli with positive MUG and positive Indole.Also,the new method could check out the E.coli with negative MUG and positive indole.So,the miss check problem of false negative and false positive results could be successfully solved.This method has the characteristics of quickness,simplicity and accuracy. The comparative experiments of both this new method and Chinese Pharmacopia to detect E.coli,have been done collaboratively by 20 provincial and municiple institutes for drug control and some pharmacy manufactories.The results indicated that:1.In 1576 batches of Chinese traditional patent medicines,45 batches were check out E.coli and 1494 batches were not checked out E.coli by both methods.The consistence of both methods to check E.coli was 97 7 % 2.It has been extensively analyzed for the unconsistence results of both methods,e.g.MI test ++ IMViC test ++-+ and MI test -- IMViC test -+--.3.When the results of IMViC test were ++-- or -+--,it might be other bacteria of non-E.coli in Escherichia.