离子对反相高效液相色谱法测定头孢三嗪、7-氨基头孢菌烷酸及7-氨基头孢烯三嗪酸的含量

Determination of Ceftriaxone(CFT),7 ACA and 7 ACT by Ion-pair RP-HPLC

用四丁基溴化铵离子对试剂，在Ｃ１８色谱柱上同时测定头孢三嗪产品，７氨基头孢菌烷酸原料及７氨基头孢烯三嗪酸中间体的含量。流动相为乙腈—四丁基溴化铵—ｐＨ７０磷酸盐缓冲液—水（３２∶０３２∶４４∶６３６），检测波长２７０ｎｍ。结果表明：７氨基头孢菌烷酸浓度在９３６～２３４μｇ／ｍＬ，７氨基头孢烯三嗪酸浓度在１０４～２６０μｇ／ｍＬ，头孢三嗪浓度在１２９２～３２３μｇ／ｍＬ的范围均存在良好的线性关系，回收率分别在９９７％～９９９％，９９５％～９８％，１０００％～１００４％。

In this paper,a reversed-phase HPLC method on ODS column with tetrabutyl ammonium bromide as ion-pair agent was developed for the quantitative analysis of CFT,7 ACA and 7 ACT.The mobile phase was acetonitrile-tetrabutyl ammonium bromide-pH 7 0 phosphate buffer-water(32∶0 32∶4 4∶63 6).The eluant was detected at 270 nm,over the concentration range 9 36～234 μg/mL of 7 ACA,10 4～260 μg/mL of 7 ACT and 12 92～323 μg/mL of CFT,respectively.The calibration curves were linear.The average recovery was the range of 99 7 %～99 9 % of 7 ACA,99 5 %～99 8 % of 7 ACT and 100 0 %～100 4 %of CFT respectively.