糖尿病中国地鼠锂代谢变化的研究

THE PRELIMINARY OBSERVATION ON LITHIUM METABOLISM IN SPONTANEOUS HEREDITARY DIABETIC CHINESE HAMSTER (SHANYI COLONY)

２０只糖尿病和１０只正常中国地鼠配对，随机分为４组：正常对照组（Ｃ）、糖尿病组（Ｄ）、正常用锂处理组（ＣＴ）和糖尿病用锂处理组（ＤＴ）。锂处理为动物饮用含碳酸锂２８．８ｍｍｏｌ／Ｌ的水，在０，１，３，５，６月测定血糖，在１，３，６月测定胰岛素水平。实验结束时处死动物取组织经湿消化后，用电感耦合等离子体－原子发射光谱仪（ＩＣＰ－ＡＥＳ）测定锂含量。同时测定血果糖胺、乳酸、ＧＰＴ和ＢＵＮ。结果：Ｄ组肝锂含量显著低于Ｃ组（Ｐ＜０．０５），肾与肌肉的锂含量也降低。ＤＴ组组织锂含量较Ｄ组高，与Ｃ组相似，且血糖与果糖胺下降，而胰岛素、乳酸、ＧＰＴ和ＢＵＮ无明显变化。实验表明糖尿病中国地鼠（山医群）有肝、肾和肌肉缺锂。小剂量６个月的锂处理能改善组织缺锂和糖代谢。

To observe the changes of tissue lithium content and its relationship with glucose metabolism in spontaneous hereditary diabetic Chinese hamsters (HSDCH), twenty diabetic and ten normal Chinese hamsters were paired and separated randomly into four groups:controls (C),diabetics (D),controls treated with lithium carbonate (CT) and diabetics treated with lithium carbonate (DT).The lithium carbonate treatment was administrated with drinking water which contained lithium carbonate (0.2mg/ml).Blood goucose levels were determined at 0,1,3,5,6th month,and insulin levels at 1,3,6th month.The lithium contents in liver,kidney and muscle were determined at the end of 6th month with wet digestion assay and ICP AES. Concentrations of fructosamine,lactic acid,GPT,BUN also were evaluated.The data showed that in Group D the lithium levels in hepatic tissue were lower than that in Group C (P<0.05),and lithium contents in kidney and muscle were also decreased;that in Group DT,the lithium contents in tissues were higher than in Group D (P<0.05) and similar to that in Group C,CT,and blood glucose levels and fructosamine concentrations decreased while insulin and lactic acid levels did not alter significantly;that the GPT and BUN levels did not change noteworthily in both Group CT and Group DT.There is lithium deficiency in hepatic,renal and muscular tissues from diabetic Chinese hamsters.Low dose and six month treatment of lithium carbonate can improve tissue lithium deficiency and improve glucose metabolism,and do not damage the liver and kidney function.