雏鸡基底乳头庆大霉素损伤后TGFβ_1、TGFβ_2、TGFβ_3的表达

Study on TGFβ_1,TGFβ_2,TGFβ_3 expression in the chick basilar papilla following gentamicin toxicity

应用免疫组化技术，检测雏鸡基底乳头庆大霉素损伤后1、3、7、14、21和28d时TGFβ1、TGFβ2、TGFβ3的表达情况。结果显示，在雏鸡基底乳头庆大霉素损伤后修复过程中，可见修复的早期TGFβ2、TGFβ3有明显表达，阳性细胞主要位于基底乳头毛细胞损伤区域的腔面细胞；TGFβ1未见阳性表达。提示，在毛细胞再生过程中TGFβ2、TGFβ3可能对移行至基底乳头腔面的细胞起着一定的调节作用。

The β-type transforming growth factors (TGFβs) are secreted proteins,which play an important role in regulation of cell proliferation and differentiation in the embryonic inner ear. In order to probe into the effect of TGFβs on the hair cell regeneration, expression of TGFβ1, TGFβ2,and TGFβ3 proteins were examined by using immunohistochemisty in the chicken basilar papilla during hair cell regeneration following gentamicin ototoxicity. Ten-day-old chickens received daily subcutaneous injection of gentamicin sulfate 50 mg/kg of ten consecutive days. The animals were allowed to survive 1, 3, 7, 14, 21 and 28 days before sacrifice and preparation for examination of the expression of TGFβ1,TGFβ2, and TGFβ3 proteins. Immunostaining results demonstrated that TGFβ2,and TGFβ3 proteins were observed in the damaged region of basilar papilla. TGFβ2, and TGFβ3, proteins positive cells were limited to the lumenal nuclear layer within the damaged region. TGFβ1, protein positive cell was not found in our study. These results indicated that TGFβ2 and TGFβ3 proteins might play a role in regulating proliferation of the supporting cells immigrated into the lumenal nuclear layer during hair cell regeneration.