双价痢疾菌苗株免疫小鼠后GALT中ASC的观测

DETECTION OF ASC IN GALT AFTER IMMUNIZATION WITH TWO BIVALENT SHIGELLA VACCINES IN MICE

为了观测两株菌苗ＦＳＭ－２１１７（Ｉｐａ－）、ＦＳ－５４１６（Ｉｐａ＋）免疫后所引起的肠粘膜相关淋巴组织（ＧＡＬＴ）中的免疫反应，以小鼠灌胃免疫为模型，应用ＢＡ－ＥＬＩＳＰＯＴ法检测了ＧＡＬＴ中诱导部位派伊尔小结（ＰＰ）、肠系膜淋巴结（ＭＬＮ）和抗原特异性抗体分泌细胞（ＡＳＣ），发现两菌苗株免疫小鼠后，ＰＰ结、ＭＬＮ中福氏、宋内氏抗原ＳＩｇＡ、ＩｇＧ抗体分泌细胞明显增加，尤以ＳＩｇＡ－ＡＳＣ为甚。株间差别无统计学意义。两株菌苗与对照组相比。

FSM 2117 and FS 5416 are two bivalent hybrid strains of S.flexneri andS.sonnei,constructed by our laboratory The FS 5416 expresses four invassive plasimid antigens(IpaA、IpaB、IpaC and IpaD)and has a good contact heamolysis activity(CHA +),but the FSM 2117 hasnt.We detect the changes of ASC in the induced sites of GALT of mice by BA ELISPOT assay after immunization with the two different vaccines,and observed that the numbers of SIgA and IgG ASC showed a significent rise in PP and MLN.Significent differences were observed between two immunized groups and the control.