维生素A对胸腺凋亡有关基因的影响

STUDY OF DOWN REGULATION OF FGFR1 ON CELL PROLIFERATION

为观察营养缺乏及补充维生素Ａ（ＶＡ）对胸腺细胞凋亡有关基因的影响，采用原位杂交及免疫组化的方法观察了胸腺组织ｂｃｌ－２ｍＲＮＡ，Ｆａｓ及Ｂａｘ蛋白的表达。结果表明营养缺乏的大鼠胸腺细胞ｂｃｌ－２ｍＲＮＡ的表达明显降低，而Ｆａｓ及Ｂａｘ蛋白的表达则明显增强；补充ＶＡ后大鼠胸腺ｂｃｌ－２ｍＲＮＡ的表达明显增强，Ｂａｘ蛋白的表达明显降低，而Ｆａｓ蛋白的表达无明显变化。本结果表明：营养缺乏时胸腺细胞凋亡增加导致免疫功能降低，补充ＶＡ可抑制细胞凋亡，可能是通过促进ｂｃｌ－２ｍＲＮＡ的表达。

Full length cDNAs of fibroblast growth factor receptor 1(FGFR1) and fibroblast growth factor receptor 1(FGFR1) were isolated from human lung fibroblasts by RT PCR.It was cloned into eukaryote expression vector pRC/CMV plasmid,respectively.Recombinant plasmid pRC/CMV FGFR1 and pRC/CMV FGFR1 were transfected respectively and cotransfected into myoblast L6 cells.FGFR1 can down regulate cell proliferation by mitogenesis in 3H TdR.