老年肿瘤患者手术前后血小板膜糖蛋白的流式细胞检测

DETECTION OF NADH CYTOCHROME b5 REDUCTASE ACTIVITY BY IMMUNO DOT ASSAY

应用流式细胞学技术对９８例老年恶性肿瘤患者手术前后外周血血小板Ｐ－选择素（ＣＤ６２ｐ），凝血酶敏感蛋白（Ｔｓ）及血小板整合素αⅡｂ链（ＧＰⅡｂ或ＣＤ４１）的变化进行了对比研究。结果显示：术后外周血ＣＤ６２ｐ、ＣＤ４１及Ｔｓ较术前明显增高，有统计学意义，其中ＣＤ６２ｐ、ＣＤ４１增加更为明显（Ｐ＜０．０１）。男性患者术后血小板活化较女性患者更为显著。实验表明：手术对老年恶性肿瘤患者血小板有明显的活化作用，以老年男性尤为显著。应重视手术后的防凝措施。

To capture b5 reductase from hemolysate, monoclonal antibody against b5 reductase was dot blotted onto nitrocellulose membrane. Spots carrying b5 reductase were activety stained with substrate consists of NADH DCIP MTT.Negative results were observed in patients of hereditary methemoglobinemia from 5 different pedigrees. In this paper, we established a simple and straightforward method for detection of NADH cytochrome b5 reductase from patient with hereditary methemoglobinemia.