线粒体蛋白Bcl-2和Bax在肿瘤发生中作用的初步探讨

A Preliminary Study on the Role of Mitochondrial Protein, Bcl-2 and Bax in Tumorigenesis

目的:研究线粒体蛋白(MAB1273)、Bcl-2、Bax在肾细胞癌(RCC)组织内的表达及相关性分析。方法:采用免疫组织化学SP方法检测9例嗜酸细胞瘤、6例嫌色细胞癌、23例透明细胞癌以及12例正常肾组织中MAB1273、Bcl-2和Bax蛋白的表达。结果:MAB 1273和Bel-2在嗜酸细胞瘤、嫌色细胞癌、透明细胞癌中表达明显高于正常肾组织(P=0.006,P=0.008),Bax在各组间表达无明显差异(P=0.057)。通过秩相关分析,MAB1273的表达与Bcl-2的表达呈正相关(r=0.341,P=0.015),而Bcl-2表达与Bax表达呈负相关(r=0.287,P=0.043)。结论:线粒体蛋白及Bcl-2的高表达、Bax低表达可能共同参与了肾嗜酸细胞瘤、嫌色细胞癌及透明细胞癌的发生,提示线粒体蛋白表达异常参与RCC细胞凋亡调控过程。

Objective： To study the expressions of mitochondrial protein （MAB1273）, Bcl-2, and Bax in renal cell carcinoma and to investigate their role in the tumorigenesis. Methods： The expressions of mitochondriai protein, Bcl-2 and Bax were detected by immunohistochemistry SP method in 9 tissue samples of renal oncocytoma, 6 samples of chromophobe carcinoma, 23 samples of clear cell carcinoma and 12 samples of normal renal tissue. Results： The expression of MAB1273 in renal oncocytoma, chromophobe carcinoma and clear cell carcinoma was much higher than that in normal renal tissue （P=0.006）. No significant difference was found in MAB1273 expression among the three types of renal carcinoma. The expression of Bcl-2 was higher in the renal carcinoma groups than in the normal renal tissue group, with a significant difference （P=0.008）. The expression of Bax was not different among all of the groups （P=0.057）. Rank correlation analysis showed a positive correlation between MAB1273 and Bcl-2 expression （r=0.341, P=0.015）. The expression of Bcl-2 was negatively correlated with Bax expression （r= -0.287, P=-0.043）. Conclusion： Overexpression of mitochondrial protein and Bcl-2 and underexpression of Bax may play a part in the genesis of renal oncocytoma, chromophobe carcinoma and the clear cell carcinoma, indicating that overexpression of MAB1273 may be correlated with apoptosis of renal cell carcinoma cells.