与苜蓿褐斑病(CLS)抗性基因连锁的SRAP标记研究

Analysis of SRAP Markers Associated with Common Leaf Spot Disease Resistance Gene in Alfalfa

【目的】寻找与苜蓿抗褐斑病基因连锁的分子标记。【方法】以褐斑病中等抗性亲本杂交组合(YL0602M×SH0602M)的F1分离群体为研究材料,采用SRAP分子标记技术,结合BSA法筛选与抗褐斑病基因连锁的分子标记。【结果】SRAP引物对Me3/Em3在抗、感病DNA池以及建池20单株中产生特异片段。测序结果显示,目标片段大小为169bp,将该标记命名为M3E3-R169。【结论】标记M3E3-R169在20个建池抗、感单株中出现的符合率为80%,初步确定其与苜蓿抗褐斑病基因连锁。

[ Objective] The study identified the molecular markers linked to common leaf spot disease resistance gene in alfalfa. [ Method ] With a F1 population produced by two middling resistant parents （YL0602M×SH0602M）, the molecular marker associated with common leaf spot disease resistance gene was screened by BSA method and SRAP technology. [Result] One specific band was found between resistant and susceptible DNA pool and 20 plants that comprised the two pools. Sequencing of the fragment indicated that its length was 169 bp. This SRAP marker was named as M3E3-R169. [Conclusion] The coincidence rate of the existence of M3E3-R169 in 20 plants was 80%, so M3E3-R169 would be linked to common leaf spot disease resistance gene.