亚胺培南和头孢他啶治疗铜绿假单胞菌败血症诱导TNF的实验研究

Experimental study on TNF induced by imipenem and ceftazidime in treatment of Pseudomonas aeruginosa septicemia

在家兔铜绿假单胞菌败血症模型上观察并比较了亚胺培南和头孢他啶单次给药诱导肿瘤坏死因子（ＴＮＦ）产生的过程、强弱及对动物存活的影响；从药物杀菌作用机制方面对结果进行了分析。亚胺培南组血清ＴＮＦ水平从第３～９小时呈现较为平稳的变化过程；头孢他啶组至第７小时出现了第二峰，明显超过对照组和亚胺培南组（Ｐ＜０．０５），其中１只动物死亡，可能与血清ＴＮＦ水平的过度升高有关；使用诱导ＴＮＦ产生较多的药物可能加重了铜绿假单胞菌败血症对机体的损害。抗生素间诱导ＴＮＦ的差异可能与药物作用于不同类型的铜绿假单胞菌青霉素结合蛋白有关。

In the rabbit model of Pseudomonas aeruginosa , we observed the profile and level of TNF induced by imipenem and ceftazidime in single dose and the effect on animal survivor. The serum TNF level was stable from the third hour to ninth hour in the imipenem group, the second TNF peak appeared in the seventh hour in the ceftazidime group and its level markedly excecded that of the control and imipenem group ( P <0.05) . One rabbit died in ceftazidime group , it might be related to the excessive elevation of TNF level. The antibiotics inducing more TNF could accelerate the lesions caused by P.aeruginosa to body. The TNF level induced by antibiotics might be related to the PBP binding sites of P.aeruginosa .