V_(79)和V_(79-8)细胞周期及其调控因子的差异比较

DIFFERENTIAL CELL CYCLE REGULATION BETWEEN V 79 AND V 79 8 CELLS

为了解Ｖ７９和Ｖ７９－８两种细胞周期及调控因子的差异，用［３Ｈ］胸腺嘧啶核苷参入法检测两种细胞，发现Ｖ７９细胞无可检测到的Ｇ２期；Ｖ７９－８细胞则同时缺乏可检测到的Ｇ１和Ｇ２期。流式细胞光度术显示，Ｖ７９Ｇ１期细胞明显多于Ｖ７９－８，表明两者在Ｇ１期调控上存在差异。比较两者Ｇ１期周期调控因子的ｍＲＮＡ表达水平发现，Ｖ７９－８ｃｙｃｌｉｎＤ１，ｃｙｃｌｉｎＤ３和Ｉｄ表达水平明显高于Ｖ７９。

V 79 and V 79 8 are two Chinese hamster lung fibroblast cell lines which proliferate very rapidly.It is verified through [ 3 H] thymidine incorporation assay that V 79 cells have non detectable G2 phase and V 79 8 cells have neither G1 nor G2 phase.With flow cytometry,we found that V 79 had obviously more G1 phase cells than V 79 8 ,which indicates that they are different in G1 phase regulation.Comparing the mRNA expression levels of several regulators in G1 phase,we also found that the expression levels of cyclin D1,D3 and Id in V 79 8 cells was obviously higher than that in V 79 cells.This might cause the G1 difference between V 79 and V 79 8 cells directly.