辣根过氧化酶在磁性纳米修饰的离子注入电极上的电化学和电催化行为

Direct electrochemistry and electrocatalysis of horseradish peroxidase at a novel magnetic nanoparticles modified NH_2/ITO ion implantation-modified electrode

辣根过氧化酶（HRP）在Co/NH2/ITO离子注入电极上有一对良好的氧化还原峰,峰电位分别为Epc=-0.2 V,Epa=-0.01 V（vsAg/AgCl）。该修饰电极对H2O2具有催化作用,可以用作H2O2的生物传感器,峰电流与H2O2的浓度分别在1.0×10-10～2.0×10-8mol/L和2.0×10-8～1.0×10-7mol/L范围内呈线性关系,线性回归方程分别为Ip（mA）=2.2986＋0.06632c（nmol/L）和Ip（mA）=3.5788＋7.3053E-4c（nmol/L）,相关系数分别为0.9972和0.9688。检出限为1.0×10-10mol/L。

With Co/NH2/ITO ion implantation modified electrode as the working electrode,a sensitive redox peak of HRP was obtained by cyclic voltammetry.The peak potentials were-0.2 V and-0.01 V（vs Ag/AgCl）.The electrocatalytic reduction of H2O2 was tested by cyclic voltammetry.The linear ranges of H2O2 at the HRP/Co/NH2/ITO electrode were 1.0×10-10～2.0×10-8 mol/L and 2.0×10-8～1.0×10-7 mol/L,respectively.The detection limit of H2O2 at the HRP/Co/NH2/ITO electrode is 1.0×10-10 mol/L.