包涵体重组蛋白的纯化及复性

Purification and Refolding of Recombinant Protein as Inclusion Bodies

综述了近年来国内、外在包涵体重组蛋白纯化及复性研究方面的主要进展．包括包涵体的处理及变性蛋白复性的各种最新技术。

With the development of genetic engineering it has become a routine procedure to produce recombinant mammalian protein from microbial cells.Now more and more genetic recombinant proteins have been cloned to the E.coli and other bacteria in Lab.But most highly expressed recombinant proteins from bacteria always form inclusion bodies,which have no native structures and no biological activities.This is the chief question that the genetic engineering is facing when it comes to the industrial production.In this paper we describe the new methods and development in the research of purification and refolding of recombinant proteins.