褐藻胶的酶法提取研究

Extraction of alginates by enzymatic hydrolysis

采用纤维素酶、果胶酶和蛋白酶酶解法提取了海带褐藻胶,并对工艺进行了优化。试验结果表明:加入海带干重的2%纤维素酶,55℃(pH值4.5)水解20min,然后加入1%果胶酶60℃(pH值4.5)浸提1.5h,再加入1%蛋白酶,80℃(pH值8.0)水解3h,接着将浸提液加热至沸腾10min钝化酶活,最后加入1mol/L氯化钙(浸提液:氯化钙=4:1(v/v),离心沉淀后即得褐藻酸钙沉淀,加入次氯酸钠漂白后经盐酸酸化生成褐藻酸,干燥脱水后加入稀碳酸钠溶液即制得褐藻酸钠。

Alginates was extracted from laminaria by hydrolysis of cellulase, pectinase and protease and its extraction processes has been optimized. The results indicated that the optimal hydrolysis conditions were： 2% cellulase （at 55℃ and pH 4.5 for 20 min）, 1% pectinase （at 60℃ and pH 4.5 for 1.5h） and 1% protease （at 80℃and pH 8.0 for 3h）. And then all the enzymes were inactived by boiling solution for 10 min. 1 mol/L CaCl2 was finally added to the hydrolysis solution with the volume ratio of 1：4 （the hydrolysis solution： CaC12 solution）. In the end, alginates was obtained by bleaching, acidification, drying and alkalization.