关于整合子在鲍氏不动杆菌多药耐药性中的作用

Integron-mediated Antibiotic Multiresistance in Acinetobacter baumannii Clinical Isolates

目的研究来源于沈阳地区的多药耐药鲍氏不动杆菌Ⅰ类整合子中耐药基因盒的携带情况。方法采用纸片扩散法筛选50株多药耐药鲍氏不动杆菌,采用PCR方法检测整合子的携带情况并对其进行DNA序列测定。结果50株菌株中有32株检测到Ⅰ类整合子,测序结果显示,共携带2种已知结构基因盒(arr-3、aacA4)。结论整合子在医院鲍氏不动杆菌不同菌株间水平传播的潜在危险性。

OBJECTIVE To determine the category of gene cassettes in type 1 integrons of multi-resistant Acinetobacter baumannii from Shenyang. METHODS Fifty clinical isolates were collected from the First Affiliated Hospital of China Medical University and K-B test was used to determine the susceptibility and the results were read based on Clinical and Laboratory Standards Institute （CLSI） of the USA. PCR was used to detect the existance of integron,and DNA sequencing was also required. RESULTS There are thirty-two isolates positive for integron. Sequencing analysis showed the integrons carried two different known genes （arr-3 and aacA4）. CONCLUSIONS The results show the potential risk of integron dissemination among different strains of A. baumannii.