红毛五加叶中常春藤皂苷元的高效液相色谱法测定

HPLC Determination of Hederagenin in Leaves of Acanthopanax giraldii Harms

目的建立红毛五加叶中常春藤皂苷元的测定方法。方法采用高效液相色谱法。色谱柱为Welchrom C18色谱柱（4．6mm×250mm，5μm）；流动相为甲醇-水-冰醋酸-三乙胺（85：15：0．04：0．02）；流速：1．0ml·min^-1；柱温：35℃；检测波长为210nm。结果常春藤皂苷元的线性范围为0．68～10．94μg，平均加样回收率为100．42％，RSD小于2％。结论该方法准确、专属性强，可为红毛五加叶的综合开发利用提供参考。

Objective To develop a determination method for the hederagenin in leaves of Acanthopanax Giraldii Harms. Methods Hederagenin was determined by HPLC method. The separation was performed on a welchrom C18 column（4.6 mm × 250 mm, 5 μm） with mobile phase of methanol - water - glacial acetic acid - triethylamine （ 85 ： 15：0.04：0.02 ）. The flow rate was 1.0 mL · min^-1. The column temperature was at 35 ℃. The detection wavelength was 210nm. Results The linear range of hederagenin was 0. 68 - 10.94 μg. The average recovery was 100.42% ,and the RSD was less than 2%. Conclusion The method is accurate and soecific which is useful for the integrative exploitation of the leaves of A. Giraldii Harms.