野葛根和银杏叶提取物配伍复方制剂的抗氧化实验研究

Experimental Study of Compound Ginaton on Antioxidation

目的观察银杏叶复方制剂对老龄小鼠的抗氧化作用。方法老龄小鼠40只,随机分成老龄对照组,银杏叶单方制剂组,银杏叶复方制剂高、低剂量组,每组10只。另10只标准体重小鼠作青年对照组。老龄对照组和青年对照组均给予等体积蒸馏水,各用药组连续灌胃给药10 d。末次给药后1 h,采血分离血清,检测超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果与老龄对照组比较,银杏叶复方制剂高、低剂量组和单方制剂组均可明显提高老龄小鼠血清中SOD活性,降低MDA含量。银杏叶复方制剂高剂量组提高SOD活性的强度大于低剂量组和单方制剂组(P<0.05)。结论银杏叶复方制剂具有抗氧化作用且呈现一定的量-效关系,处方中的银杏叶提取物与野葛根提取物成分具有一定的协同药效作用。

Objective To study the effects of compound Ginaton on SOD activity and MDA content in blood serum of aging mice. Methods 40 aging mice were randomly divided into 4 groups： aging group, positive control group （tablet Ginaton ）, high and low dose of compound Ginaton groups, 10 mice each group. Other 10 normal mice, weight of 18-22g, were taken as youth control group. Both aging group and youth control group were administered equal distilled water. After administration for 10 days, serum of each mice in all groups was separated from the whole blood of eyeball arteries, and its SOD activity and MDA contents were detected respectively. Results SOD activity in blood serum was significantly higher in positive control group, high and low close of compound Ginaton groups than that in aging control group mice. On the contrary, MDA content in blood serum of aging group was the highest among all groups. SOD activity in high dose of compound Ginaton group was significantly higher than those in low dose of compound Ginaton group and positive control group （ P 〈 0.05 ）. Conclusion Compound Ginaton has the action of antioxidation and this action has an obvious dose - effect relationship. It also shows the synergism between the two main compositions of compound Ginaton.