针刺对水液缺乏型干眼兔泪液分泌及泪腺微观形态的影响

Effect of Acupuncture on Microscopic Morphology of Lacrimal Fluid of Dry Eye Rabbit with Hydropenia

目的观察针刺对实验兔泪腺形态的影响及对应的泪液量的变化,以此来探讨针刺对泪腺微观形态的影响,从形态上直观的解释针刺增加泪流量的机理。方法选取纯种新西兰大白兔,以阿托品造模3 d成功后,分成2组,针刺组取眼周5个穴位针刺,每天1次,每次留针15 min,共针刺10 d;模型组不做处理。对比针刺对干眼模型兔泪流量及泪腺形态的影响。结果分别在实验5、71、4 d测试针刺24 h后2组泪流量。针刺组泪流量Schirme I试验(SIT)分别为:(9.79±4.39)(、11.69±4.76)、(10.36±4.57)mm/5 min;分别高于模型对照组泪流量(4.94±3.79)、(3.38±2.81)、(7.52±5.04)mm/5 min,差异有统计学意义(P<0.05)。针刺后光镜、电镜都显示泪腺形态有明显的变化,显示细胞活动旺盛。结论针刺能够改善神经反射敏感性,促进泪腺代谢,从而增加泪液的合成与分泌。

OBJECTIVE To observe the effect of acupuncture on the morphology of lacrimal fluid of experimental rabbits and the changes in lacrimal fluid volume, so as to explain the mechanism of acupuncture in increasing lacrimal fluid. METHOD Pure-bred New Zealand rabbits were made into models using atropine. Three hours later the rabbits were divided into two groups. The rabbits in the acupuncture group were given acupuncture with five points around the eye once a day with the needles retained for 15 minutes. They were treated for 10 days. No treatment was given in the model group. RESULT Lacrimal fluid volume was determined 24 hours after acupuncture treatment on the 5th, 7th and 14th day. The lacrimal fluid volume in the acupuncture group were（9.79 ± 4.39）mm/5 min, （ 11.36 ± 4.57）mm/5 min and（ 10.36 ± 4.57）mm/5 min, which were higher than the model group by（4.94 ± 3.97）mm/5 min, （3.38 ± 2.81 ） mm/5min and（7.52 ± 5.04） mm/5 min respectively, with statistical significance （ P 〈 0.05 ）. Optical and Electronic microscope showed marked morphological changes and active cell activities. CONCLUSION Acupuncture can improve neural reflex sensibility and promote lacrimal fluid metabolism, synthesis and secretion.