水中痕量微囊藻毒素测定的优化

Optimized Method for Determining Trace Levels of Microcystins in Water

文章对固相萃取(SPE)-高效液相色谱法(HPLC)测定微囊藻毒素过程中的定量参数、固相萃取柱的吸附能力、样品浓缩等进行了研究。结果表明,峰高度比峰面积在定量方面具有更好的稳定性。影响富集最大的因素是流速而不是富集次数;在藻毒素含量达到126.3μg时,仅有3.4%的藻毒素透过C18固相萃取小柱;蒸发皿蒸发可代替通用的旋转蒸发,从而优化藻毒素的测定。

This paper deals with trace determination of microcystins in water using HPLC, which involves pretreatment with solid-phase extraction （SPE） columns. Adsorption capacity of the solid phase extraction cartridges, condensation of water sample and some of the relative quantitative parameters are investigated. Result shows that peak height is of more quantitative stability than that of peak area, and the main factor affects enrichment is velocity rather than times for enrichment. To facilitate pretreatment of samples, it is suggested to use dish evaporating method in place of the common rotation evaporation.