丙型肝炎病毒核心抗原检测技术的临床应用

Clinical application of the detection technique of hepatitis C virus core antigen

目的探讨丙型肝炎病毒(HCV)核心抗原检测[酶联免疫吸附试验(ELISA)]法的临床应用价值。方法采用聚合酶链反应(PCR)-荧光探针法对临床HCV感染者血浆进行HCV-RNA检测,并用ELISA对标本进行HCV抗体和HCV核心抗原的检测。结果87例HCV感染者HCV-RNA的检出率为75%(65/87);HCV抗体的检出率为95%(83/87);HCV核心抗原的检出率为67%(58/87)。另有2例标本HCV抗体阴性,而HCV核心抗原阳性,HCV-RNA也为阳性。结论HCV核心抗原的检测对HCV感染的早期诊断有重要价值。

Objective To disscuss the clinical application value of tile method used in detection hepatitis C virus core antigen （HCVcAg） by enzyme-linked immunosorbent assay （ELISA）. Methods The HCV-RNA in the plasma samples from the people infected HCV were detected by PCR-fluorescence probing assay. The HCVeAg and HCV antibody （HCVAb） were detected by ELISA. Results The positive rate of HCV-RNA, HCVAb and HCVcAg in 87 patients were 74.7% （about 65 in 87）, 95.4% （about 83 in 87） and 66.7% （about 58 in 87）, respectively. In addition, there were two patients whose HCVAb was negative but the HCVcAg and the HCV-RNA were positive. Conclusion The detection of HCV core antigen is important in HCV infection early diagnosis.