四逆汤不同提取液对甲减阳虚证大鼠生殖器官及性激素的影响

Study on effect of various extract of Sini Decoction on reproductive organ and sexual hormone of hypothyroidism rats with syndrome of yang deficiency

目的:研究四逆汤不同提取液对甲减阳虚证大鼠模型下丘脑-垂体-性腺轴的影响,探讨四逆汤发挥助阳作用的机制与物质基础。方法:灌胃丙基硫氧嘧啶溶液法造模,用四逆汤不同提取液对此动物模型进行治疗,实验结束后,摘取生殖器官,测其质量指数并作病理切片,同时检测性激素水平。结果:四逆汤不同提取液可以对抗大鼠模型睾丸、前列腺质量指数的增加,减轻其病理损伤,且明显提高性激素睾酮水平,四逆汤醇提液疗效显著。结论:四逆汤可改善阳虚证大鼠生殖器官质量指数及性激素水平。

Objective： To study the effect of various extract of Sini Decoction on hypothalamus-pituitary-sexual glands axle of hypothyroidism rats with syndrome of yang deficiency, and discuss the mechanism of Sini Decoction in reinforcing yang. Methods： The model of deficiency of both the spleen-yang and the kidney-yang were induced by PTU solution. After the model were confirmed, the rats in Sini Decoction group were administered with Sini Decoction. At the end of the experiment, all the rats were sacrificed and the blood were sampled to check the level of Sex hormones, the reproductive organs were weighed, the testicle were sliced to check their pathological change. Results：Various extract of Sini Decoction can reduce the increase of weight of orchis and prostate of model rats, reduce their pathologic damage, and increase the level of testosterone. The alcohol extract of Sini Decoction has obvious therapetic effects. Conclusion： Sini Decoction can improve the sexual hormone level and weight index of reproductive organs of hypothyroidism rats with syndrome of yang deficiency.