黄芪注射液对奥沙利铂致海马神经细胞凋亡的抑制作用

Effect of Astragalus Root Injection on hippocampal neuronal apoptosis induced by axaliplatin

目的:研究黄芪注射液对奥沙利铂(L-OHP)致海马神经细胞凋亡的作用。方法:在原代培养的新生24hSD大鼠海马神经细胞培养液中,加入不同浓度L-OHP(0、3、5和10μg/mL)和不同浓度黄芪注射液(0.05、0.2和0.5g/mL),观察各组细胞凋亡情况,并测定细胞外液神经生长因子(NGF)含量。结果:黄芪可浓度依赖性地减轻L-OHP引起的神经细胞凋亡(P<0.01)并能增加NGF含量(P<0.05)。结论:黄芪可能通过增加NGF含量抑制L-OHP导致的海马神经细胞凋亡。

Objective： To study the effects of Astragalus Root lnjection（a traditional Chinese herb medicine） on oxaliplatin-induced apoptosis of bippocanapal neurons. Motheds： The primary culture of hippocampus neurons from SD rats （aged within 24h） were treated with different concentrations of oxaliplatin（L-OHP） （0, 3, 5 and 10μg/mL） and Astragalus Root Injection （0.05, 0.2 and 0.5 g/mL）. The apoptosis of the neurons and the nerve growth factor （ NGF ） level were analyzed. Results： Astragalus Root Injection dose-dependently decreased neuronal cell apoptosis induced by oxaliplatin （P〈 0.01） and increased NGF level （P〈0.05）.Conclusion： Astragalus Root Injection can restrain hippocampus neuronal cell apoptosis induced by oxaliplatin, probably via increasing NGF level.