532nm激光辐照对白菜幼苗维生素C含量的影响

Effects of 532 nm Laser Irradiation on Vc Content in Cabbage Seedlings

探讨532 nm激光选育高维生素C白菜的可行性。［方法］以白菜为实验材料，采用不同功率密度和不同辐照时间的Nd：YAG倍频激光对白菜种子胚进行辐照，研究激光作用方式对白菜幼苗功能叶片面积、叶绿素和维生素C含量的影响。［结果］激光辐照功率为2-20 mW/mm2时，功能叶片面积和叶绿素含量与激光辐照功率、作用时间有关，其中以14 mW/mm2激光预处理白菜种子1 min的效果最佳;辐照时间为1-5 min时，白菜幼苗维生素C含量与激光辐照剂量有关，其中以12 J （20 mW/mm2，3 min）激光预处理白菜种子效果最佳。［结论］适当辐照剂量的532 nm激光辐照白菜种子胚，可显著提高其幼苗维生素C含量。

[ Objective ] The aim was to discuss the feasibility of breeding cabbage with high Ve by 532 nm laser. [ Method ] With cabbage as sample, the embryo of cabbage seed was irradiated by frequency doubled Nd： YAG laser with different power densities and time, and the effects of laser action mode on functional leaf area, chlorophyll and Vc contents in cabbage seedlings were studied. [ Result ] The results showed that functional leaf area and chlorophyll content are related to laser power density and irradiating time when laser power density is 2 - 20 mW/mm2 , the optimal effect was observed at 14 mW/mm2 for 1 min; the content of Ve is related to dosage of laser irradiation when irradiating time ranges from 1 to 5 min and the optimal effect was observed at 12 J （20 mW/mm2 , 3min）. [ Conclusion] Irradiating the embryo of cabbage seed with proper irradiation dosage of 532 nm laser can increase its effect on the content of Vc notably.