摘要
[目的]为三叶半夏组培扩繁寻求最佳培养基配比。[方法]研究正交试验下不同激素浓度和配比对三叶半夏不同外植体诱导分化的影响。[结果]MS+1.5 mg/L6-BA+0.1 mg/LIAA+0.3 mg/LNAA为叶片诱导丛生芽的最佳激素浓度配比,MS+1.0 mg/L6-BA+0.2 mg/LIAA+0.3 mg/LNAA为叶柄诱导丛生芽的最佳激素浓度配比。生根培养基以1/2 MS+0.3 mg/L IBA效果较好。[结论]建立了三叶半夏的组培快繁体系。
[ Objective ] The aim of the study was to seek the best hormone concentration ratio about inducing clustered shoots of P. ternata. [ Method] The inducing effect of different hormone levels on P. ternata with orthogonal test was investigated. [ Result] The best hormone concentration ratio of inducing clustered shoots of P＋ ternata leaf was MS ＋ 1.5 mg/L 6-BA ＋ 0.1 μg/L IAA ＋ 0.3 mg/L NAA ,and the one of P. ternata petiole was MS ＋ 1.0 mg/L 6-BA ＋0.2 mg/L IAA ＋0.3 mg/L NAA. Rooting medium was 1/2 MS ＋0.3 mg/L IBA. [ Conclusion] Tissue culture system of P. ternata was established.
出处
《安徽农业科学》
CAS
北大核心
2010年第3期1643-1645,共3页
Journal of Anhui Agricultural Sciences
基金
河北省教育厅项目(Z2008457)
廊坊师范学院2009年度院级项目(LSZY200902)
关键词
三叶半夏
组织培养
正交试验
Pinellia ternate
Tissue culture
Orthogonal test
