慢性间歇低氧大鼠血管内皮细胞生长因子变化的临床意义

The Endothelial Cell Function in Rats with Chronic Intermittent Hypoxia

目的:探讨慢性间歇低氧(CIH)对大鼠体内内皮细胞功能的影响。方法:应用CIH大鼠模型,模拟阻塞性睡眠呼吸暂停低通气综合征(obstructive sleep apnea-hypopnea syndrome,OSAHS)睡眠中发生的慢性低氧、再氧合病理生理过程。将16只雄性SD大鼠分为2组(分别为CIH组和对照组),每组共8只。将CIH组大鼠放入自制的间歇低氧动物舱内,提供间歇低氧(最低吸入氧浓度6%～7%,最高吸入氧浓度20%～21%,维持时间5～7s);将对照组大鼠置于相同规格的间歇低氧动物舱内,给予空气脉冲供气。间歇低氧刺激总时间:每天8h(9:00～17:00),每周7d,持续5周,共持续35d。实验结束后(第36天),用酶联免疫吸附法(ELISA)测定大鼠血浆血管内皮细胞生长因子(VEGF)水平。结果:实验结束后(第36天),CIH组大鼠血浆中VEGF的浓度(101.08±27.55pg.mL-1)显著高于对照组(52.14±5.68pg.mL-1)(P<0.05)。结论:CIH大鼠体内存在明显内皮细胞功能障碍。

Objective：To investigate the endothelial cell dysfunction in rats with chronic intermittent hypoxia. Methods： To establish a chronic imtermittent hypoxia（CIH） animal model in rats, in order to mimic the intermittent hypoxia of obstructive sleep apnea-hypopnea syndrome （OSAHS） in humans, Sixteen healthy male SD rats were randomly assigned to two equal groups, the control group and the CIH group. The rats in CIH group were placed in an animal chamber subjected to CIH（nadir ambient oxygen concentration 6%-7% ,maximum ambient oxygen concentration 20%- 21 % ,Continuing respectively to 5-7 second） for 8 hours per day （from 9AM to 5PM） and the experimental tasted for 35 days. The rats in control group were placed in the same animal chamber without chronic imtermittent hypoxia, After the experiment,We measured the level of VEGF in plasma by EIASA. Results： VEGF in plasma in CIH group was higher than that in control group,101.08 ± 27.55 vs. 52.14 ± 5.68 pg/ml respectively （P〈0.05）. Conclusion： There are endothelial dysfunction in rats with CIH.