人腭扁桃体隐窝上皮结构的研究

A MORPHOLOGICAL STUDY OF THE CRYPT EPITHELIUM IN HUMAN PALATINE TONSILS

采用扫描电镜、透射电镜及光镜免疫金银法观察了31例慢性扁桃体炎患者和8例胎儿的扁桃体隐窝上皮结构及其中细胞的分布。电镜观察发现隐窝上皮表面存在三种类型微隐窝开口,其腔内有浸润细胞、异物及细菌。有三种特化上皮细胞(M细胞)覆盖于Ⅲ型微隐窝开口处,其结构与肠道M细胞相似,其数量随扁桃体炎的反复发作而减少。形态表明微隐窝是浸润细胞和外来抗原的出入口。M细胞与抗原的摄取及传递有关。光镜免疫金银法观察证明上皮浸润细胞中多数为OKT_s^+细胞,其中OKT_4^+者又占多数而OKT_s^+细胞较少,这些细胞是隐窝上皮参于免疫应答的结构基础。

Palatine tonsils from patients with recurrent tonsillitis (N = 31) and fetuses (N = 8) were used for study by SEM and TEM. The method of immunogold-silver staining ( IGSS ) was usedto study the distribution of T cell subsets in crypt epithelium under light microscope. Results showed that there were three types of microcrypt opening on the surface of the crypt epithelium. They may serve as orifices for the inter-epithelial passage of wandering cells and entrances for exogenous antigens and microorganisms. Some microcrypt openings were covered by microfold cell ( MC ) . Three types of MC were observed. With the advance of age and repeated tonsillitis MC may decrease in number. In cryptic epithelium, most of infiltrating mononuclear cells were OKT^+_3 cells with OKT_4 cells predominating, while only a few scattered T cells were OKTs cells.