噬菌体硝酸盐还原实验方法的建立与评估

Establishment and Evaluation of Nitrate Reductase Combined with Mycobacteriophage Assay

目的采用噬菌体与硝酸盐还原试验结合的技术，以期建立一种新的快速、简易、廉价的结核杆菌药敏检测方法。方法对49株结核杆菌的临床分离株以噬菌体硝酸盐还原试验（PhaB-NRA法）与改良罗氏绝对浓度法对结核杆菌进行利福平、异烟肼药物敏感性测定，以绝对浓度法作为参照标准判定PhaB-NRA法的敏感性、特异性、准确性及其可行性。结果PhaB—NRA法对利福平进行药物敏感性测定的敏感性、特异性、准确性分别为89．1％、9167％、89_8％；对异烟肼进行药物敏感性测定的敏感性、特异性、准确性分别为86．21％、90．0％、87．8％。它们与绝对浓度法的符合率分别为利福平：0．746，异烟肼：0．750。结论PhaB-NRA法简化了实验步骤，缩短了药敏检测时间，且无需昂贵的仪器，有望成为快速结核杆菌药物敏感性测定的初筛方法。

Objective To establish a rapid, inexpensive, and simple drug susceptibility test （DST） for Mycobacterium tuberculosis （M.tb） and evaluate its feasibility. Methods We used nitrate reductase combined with mycobacteriophage assay （PhaB-NRA） to test 49 clinical g.tb isolates, and the results were compared with those of PhaB-NRA and traditional absolute concentration method. Results The sensitivity, specificity, and accuracy of PhaB-NRA for rifampicin were 89.1%, 91.67%, and 89.8%; on the contrary, those of isonicotinyl hydrazide were 86.21%, 90.0%, and 87.8%, respectively. The coincidence between PhaB-NRA and traditional assay were 0.746 for rifampicin and 0.750 for isonicotinyl hydrazide. Conclusions PhaB-NRA is an inexpensive, rapid, and simple DST method. It is a promising rapid screening technique for DST of M.tb.